DNA Copy Number Calculator
DNA Copy Number Calculator
Instant Calculation
Calculates DNA copies and simulates PCR cycle amplification seamlessly.
1. DNA copy number
DNA concentration
The starting concentration of your DNA sample.
ng/µl
Template length
The total sequence length of your template.
Base weight
Standard molar weights used to calculate the molecular mass of your sequence.
DNA copies (Two-Way Sync)
Editing this automatically reverse-calculates your Concentration!
copies/µl
2. DNA copies per PCR cycle
Initial DNA copies
Automatically pulls from the Target Copies above, but you can override it manually.
copies/µl
Number of PCR cycles
The total number of amplification cycles your reaction will undergo.
DNA copies after PCR cycle(s)
Calculates total final yield. Two-way sync: type your desired final yield to reverse-calculate the needed cycles!
copies/µl
Instant calculation is ON.
Modifying any field maps properties flawlessly below.
Click to reset the workspace to blank fields.
Enter values to see the step-by-step mathematical extraction.
PCR Exponential Amplification
Plots the exponential growth of your DNA copies over the designated PCR cycles on a Log-10 scale.
Reference Constants
Standard molar weights used to calculate the molecular mass of your sequence.
| Nucleic Acid Type | Average Weight |
|---|---|
| dsDNA | 660 Da/bp |
| ssDNA | 330 Da/nt |
| ssRNA | 340 Da/nt |
